Yeast Nitrogen Base Without Amino acids

Weigh 6.7 g, add 5 g glucose to 1000 mL distilled water, dissolve thoroughly, then filter-sterilize and store ready for use. (The amount added may be adjusted according to process requirements. ) YNB medium composition (g/L): Ammonium sulfate 5. 0 g Inositol 2 000. 0 μg Copper sulfate 40. 0 μg Nicotinic acid 400. 0 μg Potassium dihydrogen phosphate 1. 0 g Boric acid 500. 0 μg Potassium iodide 100. 0 μg p-Aminobenzoic acid 200. 0 μg Biotin 2. 0 μg Magnesium sulfate 0. 5 g Ferric chloride 200. 0 μg Pyridoxine HCl 400. 0 μg Calcium pantothenate 2. 0 μg Sodium chloride 0. 1 g Manganese sulfate 400. 0 μg Riboflavin 200. 0 μg Folic acid 2. 0 μg Calcium chloride 0. 1 g Sodium molybdate 200. 0 μg Thiamine HCl 400. 0 μg Zinc sulfate 400. 0 μg