Xanthine Oxidase(XOD) Activity Assay Kit

Determination of Significance: XOD (EC 1.17.3.2) catalyzes the oxidation of hypoxanthine to xanthine and superoxide anion, which is one of the main sources of active oxygen and is also one of the key enzymes of nucleotide metabolism. XOD is mainly distributed in mammalian heart, lung, liver and other tissues. When liver function impaired, XOD is released into serum in a large amount, which has specific significance for the diagnosis of liver damage. Measurement Principle: XOD catalyzes hypoxanthine to produce xanthine and superoxide anion. Superoxide anion reacts with hydroxylamine hydrochloride to form NO 2- , and the NO 2- under the action of p-aminobenzenesulfonamide and naphthalene ethylenediamine hydrochloride is produced a red azo compound with a characteristic absorption peak at 530 nm. The color depth is linear with XOD activity. Self Provided: Spectrophotometer/Microplate reader, constant temperature foster box/water-bath, centrifuge, micro glass cuvette/96-well flat-bottom plate, adjustable transferpettor, mortar/homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.