Urine Creatinine Content Fluorometric Assay Kit

Introduction: Creatinine (Cre), the chemical formula is C4H7N3O, is a product of muscle metabolism in the human body, which is mainly excreted by glomerular filtration. Serum creatinine comes from both exogenous and endogenous sources. Exogenous creatinine is the product of meat metabolism in the body; endogenous creatinine is the product of muscle metabolism in the body. Principle: The creatinine in the sample is hydrolyzed to creatine under the catalysis of creatinine enzyme. Under the catalysis of creatinase, creatine hydrolyzes to creatine and urea. Sarcosine is oxidized to hydrogen peroxide under the catalysis of sarcosine oxidase and H2O2 reacts with the fluorescent probe under the action of the enzyme to produce a strong fluorescent product. The fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm is proportional to the H2O2 content. The kit calculates the creatinine content of the sample by measuring the amount of H2O2 produced. Reagents and Equipment Required but Not Provided: Fluorometric microplate reader, low temperature centrifuge, pipette, constant temperature incubator/metal bath, 96-well black opaque plate(provided), sealing plate film(provided), ice and distilled water.