UDP-glucose pyrophosphosphprylase(UGP) Activity Assay Kit

Determination of Significance: UDP-glucose pyrophosphorylase (UDP-glucose pyrophosphosphprylase, UGP, EC2.7.7.9) is widely distributed in nature. It catalyzes the activation of glucose before glycogen synthesis. UDP-glucose (UDPG) is synthesized from glucose-1-phosphate and UTP. UDPG is the main active enzyme form in higher plants and animals. As a glucose-based donor, it participates in the synthesis and metabolism of glycogen, sucrose, cellulose, etc. Measurement Principle: UGP can catalyze the reversible formation of glucose-1-phosphate. NADP was transformed into NADPH by phosphoglucose mutase and 6-phosphoglucose dehydrogenase. UGP activity can be reflected by the change of 340nm absorption value. Self Provided: Ultraviolet spectrophotometer/microplate reader, balance, low temperature desk centrifuge, water-bath, transferpettor, micro quartz cuvette/96-well flat-bottom UV plate, EP tube, mortar/homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.