Triose Phosphate Isomerase (TPI) Activity Assay Kit

Determination of Significance: Triose phosphate isomerase is an important glycolytic enzyme, which is widely present in animals, plants and microorganisms. Triose phosphate isomerase participates in glucose metabolism in the body. Triose phosphate isomerase plays an important role in glycolysis, gluconeogenesis, fatty acid biosynthesis and pentose phosphate metabolism. Measurement Principle: Triose phosphate isomerase converts dihydroxyacetone phosphate to glyceraldehyde 3-phosphate. Glyceraldehyde 3-phosphate and NAD generate 3-phosphoglycerate and NADH under the action of glyceraldehyde 3-phosphate dehydrogenase. The change in absorbance at 340nm reflects the activity of triose phosphate isomerase. Self Provided: Ultraviolet spectrophotometer, balance, water-bath, centrifuge, transferpettor, 1mL quartz cuvette, mortar/homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.