Transhydrogenase-2 Activity Assay Kit

Determination of Significance: Transhydrogenase (TH) is located in the inner membrane of mitochondria, also as known as Mitochondrial Respiratory Chain Complex VI. The enzyme catalyzes the mutual conversion of NADH+NADP+ and NAD++NADPH, and regulate the balance between NAD (H) and NADP (H) in mitochondria. The reverse reaction is called TH-2, which catalyzes NADPH and NAD+ to generate NADP+ and NADH. Measurement Principle: Both NADH and NADPH have characteristic absorption peak at 340 nm, therefore, the hydrogen transfer reaction catalyzed by TH cannot has a absorbance change at 340 nm. Replaces NAD+ with 3-acetylpyridyl adenine dinucleotide (APAD+, synthetic substrate), TH-2 can catalyzes APAD+ reduction to APADH and the reaction product has a characteristic absorption peak at 375 nm. In this kit, the TH-2 activity is quantified by measuring the rate of increase in light absorption at 375 nm. Self Provided: Spectrophotometer/microplate reader, water bath, desk centrifuge, water bath, adjustable transferpettor, micro quartz cuvette/96 well plate (96 UV plate), mortar/homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.