Total Cholesterol(TC) Content Assay Kit

Determination of Significance: Total cholesterol (TC) is the sum of cholesterol contained in all lipoproteins, including free cholesterol and cholesteryl esters. Measurement Principle: The enzyme esterase catalyzes the hydrolysis of cholesteryl esters to produce free cholesterol (FC) and free fatty acids (FFA), thus converting cholesteryl esters to FC; furthermore, cholesteryl oxidase catalyzes the oxidation of FC to produce 4-Cholestenone and H 2 O 2 ; finally, peroxidase catalyzes the oxidation of 4-aminoantipyrine and phenol by H 2 O 2 to produce red quinones, which have a characteristic absorption peak at 500 nm. The color shade is proportional to the TC content. Self Provided: Spectrophotometer/microplate reader, balance, low temperature table centrifuge, water-bath, micro glass cuvette/96 well flat-bottom plate, pipette, EP tube, distilled water, isopropyl alcohol. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.