Total Bilirubin (TBIL) Assay Kit

Total Bilirubin (TBIL) is the sum of direct bilirubin and indirect bilirubin. Indirect bilirubin is poorly soluble in water and cannot be excreted by the kidneys. Within hepatocytes ， it combines with glucuronic acid to form direct bilirubin; direct bilirubin is water-soluble and can be excreted through the kidneys in urine. The liver is responsible for the uptake ， conjugation ， and excretion of bilirubin. Total bilirubin serves as a crucial indicator of liver function and is the primary basis for diagnosing jaundice ， aiding in the diagnosis of liver/biliary diseases and blood disorders. Under acidic conditions ， indirect bilirubin is converted to direct bilirubin with the aid of the accelerator CTAB. Direct bilirubin reacts directly with the diazonium reagent (chlorinated diazobenzene sulfonic acid formed from p-aminobenzenesulfonic acid and sodium nitrite) to produce a purple-red azo compound. This compound exhibits a characteristic absorption peak at 530 nm. enabling the calculation of total bilirubin concentration.