Superoxide Dismutase（SOD）Activity Assay Kit （WST-1 Method）

Determination of Significance: Superoxide dismutase (SOD, EC 1.15.1.1) is a kind of metalloenzyme widely found in organism. It is an important oxygen radical scavenger and can catalytic disproportionation of superoxide anion to form H 2 O 2 and O2. SOD is not only the superoxide anion scavenging enzyme, but also the main H 2 O 2 producing enzyme, which plays an important role in the biological antioxidant system. Measurement Principle: Superoxide anion (O 2- ) is produced by xanthine and xanthine oxidase reaction system. O 2- can reduce tetrazolium-1(WST-1) to form a water-soluble yellow formazan dye, which has absorbance in 450 nm. SOD can remove O 2- and inhibit the formation of the formazan dye. The darker the yellow color of the reaction solution, the lower the SOD activity. The lighter the yellow color of the reaction solution, the higher the activity of SOD. Self Provided: Spectrophotometer/microplate reader, table centrifuge, water bath/constant temperature foster box, electronic balance, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/ homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.