Succinate Dehydrogenase(SDH) Activity Assay Kit

Determination of Significance: Succinate Dehydrogenase (SDH, EC 1.3.5.1) is widely found in animals, plants, microorganisms and cultured cells. SDH is a marker enzyme of mitochondria, which is a membrane binding enzyme located in the inner membrane of mitochondria. It is also one of the key points of respiratory electron transfer and oxidative phosphorylation. In addition, it provides electrons for the respiratory chain of various prokaryotic cells. Measurement Principle: SDH can catalyzes the dehydrogenation of succinic acid to fumaric acid. The dehydrogenation can reduce 2,6-dichlorophenol indophenol (DCPIP) under the transfer of phenazine dimethyl sulfate (PMS). 2,6-DCPIP has a characteristic absorption peak at 600 nm. The reduction rate of 2,6-DCPIP is determined by the change of absorbance at 600 nm, which represents the activity of SDH enzyme. Self Provided: Spectrophotometer, water-bath, tabletop centrifuge, adjustable pipette, mortar/homogenizer, 1 mL glass cuvette, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.