Solarbio

SP ELISA Kit

توضیحات و پروتکل‌های فنی

Solarbio ELISA kit uses the quantitative competitive enzyme immunoassay (competitive ELISA) technique. An SP antigen has been pre-coated onto a microplate; Add gradient-diluted standards and pre-diluted samples separately then add a biotin-conjugated antibody specific for SP to allow competitive binding between soluble SP in the samples/standards and the plate-coated SP for the limited antibody binding sites. After washing away any unbound substances horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. A Solution containing sulfuric acid is used to stop color development. The color intensity inversely proportional to the concentration of SP is then measurable at 450nm. You can calculate the concentration of SP in the samples by comparing the O.D. of the samples to the standard curve.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKSM-0040-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

1.25h

Reactivity:

Common Species

Detection Range:

78.125-5000 pg/ml

Recovery:

80%-120%

Sample Type:

Serum Plasma Cell Culture Supernatant

Precision:

Both intra-plate and inter-plate variation coefficients were less than 10%

Sensitivity:

46.88 pg/ml

Specificity:

Can detect SP in the sample, and there is no significant crossover with other related proteins.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

95400000 تومان