Sorbitol Content HPLC Assay Kit

Determination of Significance: Sorbitol is a sugar alcohol that can be metabolized slowly, similar to the structure of monosaccharides, which can be obtained by reducing the aldehyde group on glucose to hydroxyl group, which can be used as a chemical raw material, and is also often used in the food and pharmaceutical industry. Measurement Principle: The TG is extracted with isopropyl alcohol, then hydrolysis to glycerol and fatty acids after saponification of TG by KOH. Glycerol is oxidized by periodic acid to form formaldehyde. Condensation of formaldehyde and acetylacetone to form yellow components in presence of chloride ions. The yellow component has a characteristic absorption at 420 nm and proportional to the TG content. Self Provided: Spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, n-heptane, isopropyl alcohol, water bath, adjustable pipette, distilled water and 125 mL of empty bottle. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.