Soil Nitrate Reductase(S-NR)Activity Assay Kit

Determination of Significance: S-NR catalyzes the reduction of nitrate to nitrite in soil, which is the key enzyme of nitrate reduction in soil. Study on the activity of S-NR is of great significance for rational fertilization and reduction of nitrogen loss. Measurement Principle: S-NR catalyzes the reduction of nitrate to nitrite, NO 3- +NADH+H+→NO 2- +NAD++H2O; the generated nitrite can quantitatively generate red azo compounds with p-aminobenzenesulfonic acid and α-naphthylamine under acidic conditions; The unreacted NADH will inhibit the subsequent color reaction, and then carry out the subsequent reaction with PMS; the generated red azo compounds are 520 nm has a maximum absorption peak, which can be determined by spectrophotometry. Self Provided: Spectrophotometer, water bath, low temperature centrifuge, 1ml glass cuvette, distilled water, 30 mesh sieve (or smaller), ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.