Singlet Oxygen Sensor Green Fluorescent Probe（Cell-Permeable）

520AM Singlet Oxygen Sensor Green Fluorescent Probe is a cell-permeable fluorescent probe specifically developed for detecting intracellular singlet oxygen. This probe exhibits high selectivity toward singlet oxygen and, unlike existing fluorescent or chemiluminescent singlet oxygen detection reagents, shows no significant response to hydroxyl radicals, superoxide, or other reactive oxygen species (ROS). The indicator itself exhibits only faint blue fluorescence. Upon reacting with singlet oxygen, it releases strong green fluorescence (maximum Ex/Em wavelengths approximately 504/525 nm). Its mechanism of action involves the elimination of PET quenching groups through singlet oxygen reaction, thereby restoring fluorescence properties. Singlet oxygen can be generated through various pathways, such as dye photosensitization. In mammalian biology, as one of the reactive oxygen species, singlet oxygen is closely associated with the oxidation of low-density lipoprotein cholesterol and its subsequent cardiovascular effects. Polyphenolic antioxidants can scavenge and reduce reactive oxygen concentrations, potentially preventing such harmful oxidative effects. Ingestion of pigments that generate singlet oxygen upon photoactivation can cause severe photosensitivity reactions in skin, a problem particularly prominent in herbivorous animals. Additionally, singlet oxygen is considered a key active agent in photodynamic therapy.