Serum Vesicle Extraction Kit

1. Before formal experiments，conduct preliminary tests with several samples to optimize experimental conditions and achieve optimal results. 2. Briefly centrifuge reagents in screw-cap microtubes before opening to collect liquid from the cap and tube walls at the bottom，preventing reagent loss upon opening. 3. Do not mix with reagents from other brands，as this may compromise performance. 4. Bacterial or fungal contamination of samples or reagents，or cross-contamination between reagents，may cause erroneous results. 5. Use disposable tips，tubes，bottles，or glassware whenever possible. Reusable glassware should be thoroughly cleaned to remove detergent and sterilized before reuse. 6. After completing the experiment，all samples and utensils that have come into contact should be treated according to specified procedures. 7. The number of vesicles varies greatly among different cell types. Select the sample quantity based on actual conditions，downstream applications，and literature references. Use as many cells as possible when conditions permit. 8. When harvesting cells，ensure that dead cells do not exceed 5%. Apoptotic/dying cells release numerous vesicles of varying sizes，which may contaminate vesicles derived from viable cells during extraction. 9. For electron microscopy analysis，isolated vesicle samples must be stored on ice or at 4°C for no longer than two days. No freezing，as this may compromise electron microscopy results. Applicable samples: Serum