Seed viability Test Kit (Bromothymol Blue BTB method)

Product introduction Seed viability refers to the potential ability of seeds to germinate or the vitality of seed embryos, which determines the quality and practical value of seeds, and relates to the amount of seeds used during seeding. The commonly used method for measuring seed viability is germination experiment, that is, under suitable conditions, let the seeds absorb water and germinate, and count the percentage of germinated seeds in the number of tested seeds within a specified number of days. However, the conventional germination test method takes a long time and cannot be used for emergency needs, nor can it detect the viability of dormant seeds. The commonly used relatively rapid detection methods include triphenyltetrazolium chloride (TTC) method, bromothymol blue (BTB) method, red ink staining method, and fluorescence method. The viable germ embryo has respiration, which absorbs oxygen in the air and emits carbon dioxide, which dissolves in water to form carbolic acid, and then dissociates hydrogen ions and bicarbonate, which increases the acidity of the surrounding environment of the germ embryo. Therefore, bromothymol blue (BTB) can be used to determine the change in acidity. The seeds were yellow in acidic medium, blue in alkaline medium, and green in the middle color (color change point = 7.1), so the viability of seeds could be judged according to the difference of BTB color. This method has the following advantages: fast, accurate; The color changes are obvious and easy to observe. Precautions The kit is only used in the field of scientific research, not for clinical diagnosis or other purposes.