SD-Trp-Leu

Product introduction 1, Product name: Yeast defect medium SD-Trp-Leu 2, English name: SD-Trp-Leu 3, product properties: This product is white powder, odorless or slightly smelly 4, solubility: distilled water, 8g/L concentration Yeast is a commonly used model organism, often used for genetic modification, and the yeast amino acid defect medium is the necessary medium for yeast transformation and yeast hybridization, its main use: for the experimental research of yeast single hybridization/yeast double hybridization, and the screening of yeast genetic mutant strains. According to the type of defective amino acids, it is divided into four categories of media, respectively, one deficiency, two deficiency, three deficiency, four deficiency media, the company can customize the relevant media according to your requirements, quality assurance. Di-deficiency medium: This type of yeast SC-selective medium -SD medium is used for the conversion screening of two-plasmid yeast. Uses: expression of foreign genes in yeast, functional complementarity of heterologous genes, initial plasmid transformation in yeast single-hybrid system and yeast two-hybrid system. Coimmunoprecipitation, Y187 and AH109 yeast mating experiments, zygotic diploid screening. For example: SD/-Trp-Leu, SD/-Trp-His, SD/-Trp-Ade, SD/-Leu-Ade, SD/-His-Ade, SD/-Leu-His, SD/-Ura-Trp, SD/-Ura-Leu, SD/-Ura-His, SC-Trp-Leu, SD/ -trP-LEu, SD/ -Ura-LEu, SD/ -ura-his, sc-trP-LEu, SC-Trp-His, SC-Trp-Ade, SC-Leu-Ade, SC-His-Ade, SC-Leu-His, SC-Ura-Trp, SC-Ura-Leu, SC-Ura-His Precautions 1: The medium contained all the desired ingredients (except glucose, since some researchers need to use other sugars such as galactose to induce GAL1 promoter expression), a fine mixture of various amino acids and yeast nitrogen base/yeast nitrogen source, weighed at 8g/L, and added to water to make the corresponding medium. There is no need to purchase MinimalSDBase or other yeast nitrogen components. Autoclaving with sterile glucose to a final concentration of 2% (or raffinose) or the specific inducer required for the experiment (such as galactose used in the GAL1 promoter to induce expression at a dose or concentration of 2%) is suitable for the expression of foreign genes in yeast. Selective deficient cultures such as yeast yeastone-Hybrid and yeasttwo-Hybrid systems are used to screen for yeast phenotypes containing specific marker genes. 2: When yeast deficient medium is prepared, glucose can be added first followed by high pressure without serious effect on yeast growth. However, if glucose is added and then sterilized by high pressure, the medium will change color, ranging from light yellow to dark brown, which still has a certain effect on the growth of cells (but will not affect the experiment in general). Recommendation: Because amino acids and GLUCOSE can react chemically at high temperature and pressure, the Technical department does not recommend adding glucose to the post pressure, but recommends the method of the above tip 1. 3 Recommendation: raffinose and galactose cannot be exposed to high temperature and high pressure in principle, because their chemical structure will change under high temperature and high pressure, which will inhibit the induction effect. This point is especially important for researchers to pay attention to, especially when performing functional complementation experiments mediated by expression regulation. 4: When the plate is prepared, the soft Agar semi-solid medium (Agar powder is 2%, that is, 2 grams of Agar powder per 100ml) is used for transformation screening, the pH needs to be adjusted to about 6. 0 before high pressure (for convenience, the pH range can be changed between 5. 6 and 6. 5), while the liquid medium does not need to be adjusted. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.