SCDLP Broth with Polycolistin B

Principle: Casein peptone and soy peptone provide carbon sources, nitrogen sources and growth factors required for bacterial growth; Glucose is a fermentable sugar; Sodium chloride maintains a balanced osmotic pressure in the medium; Potassium monobasic phosphate is used as a pH buffer for the medium; Lecithin and Tween 80 can neutralize preservatives and can play the function of dispersing substances in the emulsion, lecithin can neutralize quaternary ammonium salts, Tween 80 can neutralize phenol, hexachlorophenol, formalin, and the combination of the two can neutralize ethanol; Polymyxin B mainly inhibits the growth of most gram-negative bacteria. Usage: Weigh 31. 0g of this product, and take Tween-80 7. 0g Heat and dissolve in 1000ml of distilled water, divide and autoclave at 121 ℃ for 20 minutes, cool, before use, add 25000U of filtered and sterilized polymyxin B per 100ml of medium, mix well, and set aside.