RNase III

1.RNA is extremely susceptible to degradation. Avoid exposure to environmental RNase throughout all operations.2.Keep RNase III on ice during use，and return it to storage at -20 °C immediately after use.3.The dosage of RNase R and reaction volume shall be adjusted according to practical experimental conditions.5.This product is for research use only. It is not intended for clinical diagnosis or therapeutic applications. Ribonuclease III (RNase III) is a double-stranded RNA (dsRNA)-specific ribonuclease derived from Escherichia coli. It is incapable of hydrolyzing DNA or single-stranded RNA. In the presence of manganese ions (Mn2?)，RNase III specifically cleaves long dsRNA into small interfering RNAs (siRNAs) of approximately 18-25 base pairs (bp). These siRNAs possess a 3’-hydroxyl terminus with 2 to 3 overhanging bases. The cleavage products are structurally similar to those generated by Dicer，and this enzyme is widely applied in researches including RNA interference，gene expression regulation，gene silencing and target validation.