Restriction Endonuclease XmnI

When using CutOne® Buffer for XmnI digestion, the digestion time should not exceed 1 hour;
The enzyme volume added to the reaction system should not exceed 10% of the total volume to avoid excessive glycerol in the enzyme causing star-shaped activity;
If the additives (e.g., glycerol, salt) in the restriction endonuclease storage buffer are the same as the contaminants (e.g., salt, EDTA, or ethanol) in the substrate solution, the smaller the reaction volume, the stronger the inhibitory effect of the digestion reaction;
Isolytic enzymes may have different sensitivities to different methylation modifications;
Inactivation conditions: Incubate at 80℃ for 20 min. XmnI is a Type IIP restriction enzyme derived from Xanthomonas manihotis, and is purified after recombinant expression in Escherichia coli. XmnI recognizes and cleaves the sequence GAANN/NNTTC, producing blunt ends after cleavage. It exhibits optimal performance and effective control of star activity in its dedicated buffer. other restriction enzymes. However, prolonged digestion should be avoided to prevent star activity. Concentration: 10?U/μLIsoschizomers: PdmI, MroXIRecognition site: GAANNNNTTC5 '…GAANN↓NNTTC…3 '3 '…CTTNN↑NNAAG…5 ' Definition of enzyme activity: One unit (U) is defined as the amount of enzyme required to completely digest 1?μg of λDNA in a 50?μL reaction system at 37?°C within 1 hour.