Restriction Endonuclease SwaI

The volume of enzyme added to the reaction system should not exceed 10% of the total volume to avoid excessive glycerol in the enzyme causing star activity;
The additives (e.g., glycerol, salt) in the restriction endonuclease storage buffer are the same as the contaminants (e.g., salt, EDTA, or ethanol) in the substrate solution. The smaller the reaction volume, the stronger the inhibitory effect of the enzyme digestion reaction;
No star activity was observed after 3 h incubation, but star activity may appear after delayed digestion;
Isolytic enzymes may have different sensitivities to different methylation modifications;
Inactivation conditions: Incubation at 80℃ for 20 min. SwaI is a Type IIP restriction enzyme derived from Staphylococcus warneri (B. Frey), obtained by recombinant expression in Escherichia coli. SwaI recognizes and cleaves the 8-base palindromic sequence ATTTAAAT, producing blunt ends. It is commonly used in molecular cloning, genotyping and other research applications. Concentration: 10?U/μLIsoschizomers: SmiIRecognition site: ATTTAAAT5 '…ATTT↓AAAT…3 '3 '…TAAA↑TTTA…5 'Definition of enzyme activity:One unit (U) is defined as the amount of enzyme required to completely digest 1?μg of plasmid V in a 50?μL reaction system at 37?°C within 1 hour.