Restriction Endonuclease PciI

The enzyme volume added to the reaction system should not exceed 10% of the total volume to avoid excessive glycerol in the enzyme causing star-shaped activity; Additives (e.g., glycerol, salt) in the restriction endonuclease storage buffer are the same as contaminants (e.g., salt, EDTA, or ethanol) in the substrate solution; the smaller the reaction volume, the stronger the inhibitory effect of the enzyme digestion reaction; PciI can be double-digested with other LightNing® series restriction enzymes using QuickCut series Reaction Beffer buffer, but long-term reactions (more than 3 h) are not recommended; Shearing may be inhibited for DNA methylated with EcoKI; Shearing may be inhibited for DNA methylated with EcoBI; Isolytic enzymes may have different sensitivities to different methylation modifications; Inactivation conditions: Incubate at 80℃ for 20 min. PciI is a Type IIP restriction enzyme derived from Planococcus citreus, and is obtained through recombinant expression in Escherichia coli. PciI achieves rapid digestion using its dedicated reaction buffer. It is compatible with the QuickCut series Reaction Buffer for double digestion, but star activity may occur during prolonged incubation. Concentration: 10?U/μLIsoschizomers: PscIRecognition site: ACATGT5 '…A ↓ CATGT…3 '3 '…TGTAC ↑ A…5 'Definition of enzyme activity:One unit (U) is defined as the amount of enzyme required to completely digest 1?μg of p616 DNA in a 50?μL reaction system at 37?°C within 1 hour.