Restriction Endonuclease BstXI

The volume of enzyme added to the reaction system should not exceed 10% of the total volume to avoid excessive glycerol in the enzyme causing star-shaped activity;
The additives (e.g., glycerol, salt) in the restriction endonuclease storage buffer are the same as the contaminants (e.g., salt, EDTA, or ethanol) in the substrate solution. The smaller the reaction volume, the stronger the inhibitory effect of the enzyme digestion reaction;
For DNA methylated by Dcm, cleavage may be inhibited;
Inactivation conditions: Incubate at 80℃ for 20 min. BstXI is a Type IIP restriction enzyme that recognizes a palindromic sequence. An optimized reaction buffer allows BstXI to function optimally; meanwhile ， the buffer contains recombinant albumin ， which enhances the stability of various enzymes. Concentration: 10?U/μLRecognition site: CCANNNNNNTGG5 ‘…CCANNNNNN↓NTGG…3 ‘3 ‘…GGTN↑NNNNNACC…5 ‘Definition of enzyme activity:One unit (U) is defined as the amount of enzyme required to completely digest 1?μg of λDNA in a 50?μL reaction system at 37?°C within 1 hour.