Reduced Glutathione(GSH) Content Assay Kit

Determination of Significance: Glutathione is a natural tripeptide composed of glutamic acid (Glu), cysteine (Cys) and glycine (Gly). It is a kind of compound containing sulfhydryl group (-SH), which widely exists in animal tissue, plant tissue, microorganism and yeast. Measurement Principle: Glutathione can react with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) to produce 2-nitro-5-mercaptobenzoic acid and glutathione disulfide (GSSG). 2-nitro-5-mercaptobenzoic acid is a yellow product with the maximum absorption at 412 nm. Self Provided: Analytical balance, mortar/homogenizer, low temperature centrifuge, water bath, adjustable pipette, spectrophotometer/microplate reader, micro glass cuvette or 96 well flat-bottom plate and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.