Red Rapid Plasmid Mini Isolation Kit

This kit adopts an improved SDS-alkaline lysis procedure. Under high-salt and low-pH conditions，plasmid DNA specifically binds to the silica membrane of the spin column. Impurities are then removed with wash solution，and pure plasmid DNA is finally eluted from the silica membrane using elution buffer. The following protocol is applicable to plasmid extraction from 1-4 mL of overnight-cultured Escherichia coli. The yield and quality of extracted plasmids are affected by host strain，culture conditions，cell lysis efficiency，plasmid copy number，plasmid stability and antibiotics. Plasmids isolated with this kit feature high yield and high purity，and can be directly used for restriction digestion，transformation，PCR，in vitro transcription，DNA sequencing and other molecular biology experiments. Product Advantages: Rapid: The entire extraction process takes only 6 minutes. Efficient: The proprietary buffer system significantly enhances plasmid DNA extraction efficiency. Visual guidance: A special color indicator monitors the whole operation process.