Solarbio

Rat TNF-α(High Sensitivity) ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Rat TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any Rat TNF-α present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for Rat TNF-α is added to detect the captured Rat TNF-α protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKR-0009(HS)-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

3.5h

Reactivity:

Rat

Detection Range:

1.95 pg/ml-125 pg/ml

Recovery:

80%-120%

Sample Type:

Serum, plasma, Cell culture supernatant

Precision:

Coefficient of variation is < 10%

Sensitivity:

0.975 pg/ml

Specificity:

Do not react with Human TNF-α,TNF-β porcine TNF-α Mouse TNF-α ect.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

130200000 تومان