Solarbio

Rat TIM-1/KIM-1/HAVCR ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TIM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any TIM-1 present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for TIM-1 is added to detect the captured TIM-1 protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKR-0145-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃,6 months

Assay Time:

3.5h

Reactivity:

Rat

Detection Range:

62.5-4000 pg/ml

Recovery:

80%-120%

Sample Type:

Serum, plasma, Cell culture supernatant

Precision:

Coefficient of variation is < 10%

Sensitivity:

31.25 pg/ml

Specificity:

Do not react with mouse TIM-1 TIM-3/Fc Chimera TIM-4 人TIM-1 TIM-4 NKp44/Fc Chimera etc.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

80400000 تومان