Rat Pulmonary Aortic Smooth Muscle Cell Isolation and Culture Kit

(1) Before the formal experiment, it is recommended to use 1-2 normal rats for anatomical simulation training to familiarize yourself with the operational procedures and improve tissue separation efficiency; (2) Reagent preparation or aliquoting must strictly adhere to aseptic technique guidelines. After aliquoting, immediately seal the containers with sealing film, use immediately upon removal, and avoid repeated freeze-thaw cycles or contamination. The Rat Pulmonary Aortic Smooth Muscle Cell Isolation and Culture Kit is specifically designed for the isolation of primary rat pulmonary aortic smooth muscle cells. Upon verification, following the standard operating procedures of this kit, one test can yield one bottle of cells (T25 culture flask), with a cell count exceeding 1×10? cells. When passaged at a 1:2 ratio, the cells can be passaged for about 5 generations, with optimal condition within 3 generations. α-SMA Immunofluorescence analysis confirms a cell purity exceeding 90%.