Rat Peripheral Blood NK Cell Isolation Solution Kit

1.It is advisable to avoid using plastic products made from highly polymerised materials (such as polystyrene) in this experiment. Instead， use non-static or low-static centrifuge tubes and glassware that has not been treated with alkali， as static electricity can cause cells to adhere to the surface， whilst alkali-treated glass surfaces become rough， thereby affecting cell separation efficiency. 2.Better separation results are achieved when the volume of the separation solution exceeds that of the blood sample.3.Some C57 mice are not suitable for use with this kit， as they are prone to incomplete erythrocyte sedimentation or even a complete failure of sedimentation; please exercise caution when selecting them. 4.Throughout the entire process， samples， reagents and the experimental environment must be maintained at 20±2°C (reagents must be brought to room temperature; 20°C in summer and 23°C in winter). 5.To obtain the best experimental results， it is advisable to carry out the experiment within two hours of sampling; the longer the sample is left to stand， the poorer the cell separation will be. If the sample is left for more than six hours， the separation will be even poorer， or may not achieve the desired result at all. This kit is a sterile， low-endotoxin density gradient separation system specifically designed for the efficient isolation of NK cells from rat peripheral blood. Based on the principle of Ficoll density gradient centrifugation， it utilises the density differences between various blood cells to rapidly isolate high-purity， highly active NK cells. The entire process is carried out under sterile conditions and is tailored to the characteristics of rat blood， providing a reliable cell preparation solution for research in immunology， oncology and inflammation.