Solarbio

Rat IL-6 ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any IL-6 present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for IL-6 is added to detect the captured IL-6 protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKR-0005-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

3.5h

Reactivity:

Rat

Detection Range:

39.06-2500 pg/ml

Recovery:

80%-120%

Sample Type:

Serum Plasma Cell Culture Supernatant

Precision:

Both intra-plate and inter-plate variation coefficients were less than 10%

Sensitivity:

19.53 pg/ml

Specificity:

Do not react with Rat GDNF&GM-CSF&IFN-γ&IL-1&IL-2&IL-4&IL-10&IL-18&PDGF-BB&TNF-α,Human IL-6&IL-6 R etc

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

80400000 تومان