Solarbio

Rat IL-4(Low Sample Volume) ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any IL-4 present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for IL-4 is added to detect the captured IL-4 protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKR-0004(L)-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

3.5h

Reactivity:

Rat

Detection Range:

23.44-1500 pg/ml

Recovery:

80%-120%

Sample Type:

Serum, plasma, Cell culture supernatant

Precision:

Coefficient of variation is < 10%

Sensitivity:

125 pg/ml

Specificity:

Do not react with Rat IL-1a,2,6,10,18,IFN-γ,TNF-α 人IL-4,IL-4R ect.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

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