Rat Hepatic Sinusoidal Endothelial Cell Isolation and Culture Kit

(1) Before the formal experiment, it is recommended to use 1-2 normal rats for anatomical simulation training to familiarize yourself with the operational procedures and improve tissue separation efficiency; (2) Throughout the entire tissue separation process, it is advisable to place the tissue-containing dishes on an ice tray/ice box (2-8°C) to maintain a low temperature. However, avoid freezing due to excessive cold, as this could cause tissue damage and impair extraction efficacy; (3) Reagent preparation or aliquoting must strictly adhere to aseptic technique guidelines. After aliquoting, immediately seal the containers with sealing film, use immediately upon removal, and avoid repeated freeze-thaw cycles or contamination. (4) During storage, the separation solution in this kit may exhibit crystal particle precipitation at the bottle opening or become turbid, which are normal phenomena and can be used with no worries. The Rat Hepatic Sinusoidal Endothelial Cell Isolation and Culture Kit is specifically designed for the isolation of primary rat hepatic sinusoidal endothelial cells. Upon verification, following the standard operating procedures of this kit, one test can yield one bottle of cells (T25 culture flask), with a cell count exceeding 1×10? cells. When passaged at a 1:2 ratio, the cells can be passaged for 1-2 generations, with optimal condition within 3 generations. Immunofluorescence analysis confirms a cell purity (CD31/CD14 positivity rate) exceeding 90%.