Rat Heart Microvascular Endothelial Cell Isolation and Culture Kit

(1) Before the formal experiment, it is recommended to use 1-2 normal rats for anatomical simulation training to familiarize yourself with the operational procedures and improve tissue separation efficiency; (2) Throughout the entire tissue separation process, it is advisable to operate on ice, but be careful not to make the tissue or liquid freeze;(3) The culture medium contains nutrients required for microbial growth. Please open it in a clean bench, aliquot and sealing the bottle opening with sealing film. Quick open and quick use, to avoid contamination; (4) During storage, the separation solution in this kit may exhibit crystal particle precipitation at the bottle opening, which are normal phenomena and can be used with no worries. This product is specifically designed for the isolation of primary rat heart microvascular endothelial cells. It employs a two-step digestion method to digest tissue and density gradient centrifugation to purify cells. Laboratory validation has confirmed that it can isolate over 1×10? cells, with an immunofluorescence identification rate (CD31 positivity rate) exceeding 90%.