Rat Cortical Neuron Cell Isolation and Culture Kit

(1) Before the formal experiment, it is recommended to use 1-2 normal rats for anatomical simulation training to familiarize yourself with the operational procedures and improve tissue separation efficiency; (2) Throughout the entire tissue separation process, it is advisable to place the tissue-containing dishes on an ice tray/ice box (2-8°C) to maintain a low temperature. However, avoid freezing due to excessive cold, as this could cause tissue damage and impair extraction efficacy; (3) Reagent preparation or aliquoting must strictly adhere to aseptic technique guidelines. After aliquoting, immediately seal the containers with sealing film, use immediately upon removal, and avoid repeated freeze-thaw cycles or contamination. (4) Cortical neuronal cells are terminally differentiated cells that cannot proliferate and are relatively fragile. Improper digestion may cause cell damage. When seeding, please calculate the seeding volume according to experimental requirements and seed into the corresponding culture vessels. Corresponding ratios: 1 T25 flask ≈ 1 6cm dish ≈ 2 6-well plate wells≈ 5-5 12-well plate wells. The Rat Cortical Neuron Cell Isolation and Culture Kit is specifically designed for the isolation of primary rat cortical neuron cells. Upon verification, following the standard operating procedures of this kit, one test can yield one bottle of cells (T25 culture flask), with a cell count exceeding 1×10? cells. Immunofluorescence analysis confirms a cell purity (β-Tubulin III) exceeding 90%.