Raka-Ray Medium

Principle: Yeast infusion，tyropeptone and liver infusion provide nitrogen sources，vitamins and growth factors; Glucose，maltose and fructose provide carbon sources; Potassium phosphate dibasic is used as a buffer; Ammonium citrate，magnesium sulfate，manganese sulfate，Tween-80 and N-acetylglucosamine provide growth factors for the cultivation of various lactic acid bacteria; The low pH of this medium inhibits the growth of other bacteria and is conducive to the isolation of lactobacilli. Agar is a coagulant of the culture medium. To increase selectivity，2-phenylethanol and cycloheximide can be added，which can inhibit gram-negative bacteria and yeasts. Usage: Weigh 74. 9 grams of this product，weigh 10 grams of Tween 80，heat and stir to dissolve in 1000ml distilled water，divide into triangular bottles，and autoclave at 121 °C for 15 minutes for later use.