QuickCut Fast Restriction Endonuclease Tsp45I

For DNA methylated by EcoBI, cleavage may be inhibited;
No asterisk activity was observed after 3 h incubation, but asterisk activity may appear after longer digestion;
Isolytic enzymes may have different sensitivities to different methylation modifications;
Inactivation conditions: Incubation at 80℃ for 20 min. QuickCut Fast Restriction Enzymes are a series of genetically engineered recombinant restriction enzymes designed for rapid digestion of plasmid DNA ， PCR products ， genomic DNA and other DNA samples. All QuickCut Fast Restriction Enzymes show excellent activity in Reaction Buffer or Reaction Color Buffer ， and can complete digestion within 5–15 minutes. Reaction Color Buffer contains red and yellow tracking dyes ， allowing digested products to be directly used for gel electrophoresis. In a 1% agarose gel ， the migration rate of the red dye in Reaction Color Buffer is similar to that of a 2500 bp double-stranded DNA fragment; the migration rate of the yellow dye is similar to that of a 10 bp double-stranded DNA fragment. Concentration: 1 rxn/μLIsoschizomers: NmuCI ， TseFIRecognition site: GTSAC5 ‘…↓GTSAC…3 ‘3 ‘…CASTG↑…5 ‘