QuickCut Fast Restriction Endonuclease SdaI (SbfI)

No asterisk activity was observed after 3 hours of incubation; longer digestion may result in asterisk activity.
Isolytic enzymes may have different sensitivities to different methylation modifications.
Inactivation conditions: Incubation at 80℃ for 20 minutes. QuickCut Fast Restriction Enzymes are a series of genetically engineered recombinant restriction enzymes designed for the rapid digestion of plasmid DNA ， PCR products ， genomic DNA ， and other DNA samples. All QuickCut Fast Restriction Enzymes show excellent activity in Reaction Buffer or Reaction Color Buffer ， and can complete digestion within 5–15 minutes. Reaction Color Buffer contains red and yellow tracking dyes ， allowing the digested products to be directly used for gel electrophoresis. In a 1% agarose gel ， the migration rate of the red dye in Reaction Color Buffer is similar to that of a 2500 bp double?stranded DNA fragment; the migration rate of the yellow dye is similar to that of a 10 bp double?stranded DNA fragment. Concentration: 1 rxn/μLIsoschizomers: AcvI ， Eco72I ， PspCI ， BbrPIRecognition site: CACGTG5 ‘…CAC↓GTG…3 ‘3 ‘…GTG↑CAC…5 ‘