Pyruvate Dehydrogenase(PDH) Activity Assay Kit

Determination of Significance: PDH（EC 1.2.4.1） widely exist in animals, plants, microorganism and cultured cells, which is the rate-limiting enzyme of acetylformic acid oxidative and decarboxylate catalyzed by Pyruvate dehydrogenase complex (PDHC). The decarboxylation of acetylformic acid forms hydroxyethyl-TPP, links glycolysis to the three carboxylic acid cycle. Measurement Principle: PDH catalyzes the dehydrogenation of acetylformic acid and reduct 2, 6-dichlorophenol indophenol (2,6-DCPIP), which makes the absorption of 605 nm decrease. Self Provided: Spectrophotometer/microplate reader, water bath, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.