Pyruvate Decarboxylase(PDC) Activity Assay Kit

Determination of Significance: Pyruvate Decarboxylase (PDC) exists in yeast mainly, which is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylation to form acetaldehyde. Measurement Principle: Ethanol dehydrogenase (ADH) is added to further catalyze the reduction of acetaldehyde by NADH to produce ethanol and NAD+. NADH has a absorbance at 340 nm but NAD + not, the activity of PDC can be calculated by measuring decrease rate of absorption at 340 nm. Self Provided: Spectrophotometer, Water-Bath/Constant Temperature Incubator, Desk Centrifuge, Adjustable Pipette, 1 mL Quartz Cuvette, Mortar/Homogenizer/Cell ultrasonic Crusher, Ice and Distilled Water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.