Pyrophosphate: Fructose-6-Phosphate-1-Phosphoric Acid Transferase(PFP) Activity Assay Kit

Determination of Significance: Pyrophosphate: Fructose-6-phosphate-1-phosphotransferase (PFP, EC2.7.1.90) is a cytosolic enzyme that is widely present in plant tissues and Catalyzes the phosphorylation of fructose-6-phosphate like phosphofructokinase. As a result, the single PEP catalytic reaction is a reversible reaction, and pyrophosphate is used instead of ATP, which plays an important role in carbon metabolism of photosynthesis. Measurement Principle: PFP catalyzes the conversion of fructose 6-phosphate to fructose 1,6-diphosphate, which is converted to dihydroxyacetone phosphate by the action of aldolase and triose phosphate isomerase, and then catalyzed by α-phosphate glycerol dehydrogenase and NADH to from Glycerol 3-diphosphate and NAD. The change in absorbance at 340 nm reflects the level of PFP activity. Self Provided: Low temperture centrifuge, spectrophotometer, water bath/constant temperature incubator, mortar/homogenizer, 1 mL quartz cuvette, transferpettor, ice and distilled water, EP tube. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.