PYG Broth Base

Product Introduction: Used for bifidobacterium growth culture. One 2. 5mg solution of heme chloride and one 1mg solution of vitamin K1 were added to every 100ml medium. Preparation Method: Weigh 18. 04g of this product, heat it and dissolve it in 500ml distilled water, autoclave it at 121℃ for 15 minutes, cool it to about 50℃, add 0. 5ml vitamin K1 solution filtered for sterilization and 2. 5ml heme chloride solution (5mg/ml), mix well and set aside. Composition: g/L Peptone: 20 Glucose: 5. 0 Yeast Infusion powder 10. 0 Sodium chloride: 0. 08 Calcium chloride: 0. 008 Magnesium sulfate: 0. 008 Potassium hydrogen phosphate: 0. 04 sodium bicarbonate: 0. 4 Cysteine hydrochloride 0. 5 potassium dihydrogen phosphate 0. 04pH: 6. 0±0. 1, 25℃ Precaution: Preparation of vitamin K1 solution: weigh vitamin K11g, add anhydrous ethanol 99ml, filter and remove bacteria. Preparation of heme chloride solution: Weigh 0. 5g heme chloride and dissolve in 1mol/L sodium hydroxide solution 1ml plus distilled water to 100ml, autoclave at 121℃, and store in refrigerator. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.