کیت
Solarbio

Progesterone ELISA Kit

توضیحات و پروتکل‌های فنی

Solarbio ELISA kit uses the quantitative competitive enzyme immunoassay (competitive ELISA) technique. An Progesterone antigen has been pre-coated onto a microplate; Add gradient-diluted standards and pre-diluted samples separately, then add a HRP-conjugated antibody specific for Progesterone to allow competitive binding between soluble Progesterone in the samples/standards and the plate-coated Progesterone for the limited antibody binding sites. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. A Solution containing sulfuric acid is used to stop color development. The color intensity, inversely proportional to the concentration of Progesterone, is then measurable at 450nm. You can calculate the concentration of Progesterone in the samples by comparing the O.D. of the samples to the standard curve.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKSM-0074-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃ 6 months
Assay Time:
2h
Reactivity:
Mammals
Detection Range:
0.0625-4 ng/ml
Recovery:
80%-120%
Sample Type:
Serum, plasma, Cell culture supernatant
Precision:
Coefficient of variation is < 10%
Sensitivity:
0.031 ng/ml
Specificity:
No cross-reactivity was observed with Progesterone-related analogs.
تحویل:
یک ماه پس از سفارش
80400000
80400000 تومان