Porcine TNF-α ELISA Kit

Solarbio ELISA kit employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Porcine TNF-α has been pre-coated onto a microplate; Add gradient-diluted standards and pre-diluted samples separately and fully combined with the specific antibody; After washing, biotinylated anti-Porcine TNF-α antibodies are added, which specifically bind to the standard and sample captured by the antibody on the plate and Porcine TNF-α in the sample; After washing, Streptavidin- Horseradish Peroxidase (HRP) conjugate was added, and biotin and streptavidin would undergo high-intensity non-covalent binding; After washing, add Substrate Solution TMB. If there are different concentrations of Porcine TNF-α in the sample in the reaction well, HRP will make the colorless TMB become a blue substance of different shades (positively correlated), and the reaction well will turn yellow after adding the stop solution; Finally, the wells are read at 450 nm, The OD value is proportional to the concentration of Porcine TNF-α. You can calculate the concentration of Porcine TNF-α in the samples by comparing the OD of the samples to the standard curve.