Solarbio

Porcine BDNF ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for BDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any BDNF present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for BDNF is added to detect the captured BDNF protein in the sample. Following a wash to remove any unbound combination,? horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKP-0052-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

2.33h

Reactivity:

Porcine

Detection Range:

46.87 pg/ml-3000 pg/ml

Recovery:

80%-120%

Sample Type:

Serum, plasma, Cell culture supernatant

Precision:

Coefficient of variation is < 10%

Sensitivity:

23.43 pg/ml

Specificity:

Do not react with BMP1 BMP2 BMP3 BMP4 IL-2 IL-4 IL-5 IL-6 IFN-γ TGFβ1 TGFβ2 TGFβ3 TLR1 TLR2 TLR4 TNF-α ect.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

80400000 تومان