Pluripotent Stem Cell Immunofluorescence Assay Kit(TRA-1-81, Mouse Anti, Green Fluorescence)

The murine monoclonal antibody in this kit is a special type of immunoglobulin, please note that it can be used in conjunction with the secondary antibody goat anti-mouse 555 in this kit, and conventional Alexa Fluor 555-labeled goat anti-mouse IgG cannot be used with this kit. The fixative solution is harmful to the human body, so please be especially careful when handling it and pay attention to effective protection to avoid direct contact with the human body or inhalation into the body. When immunofluorescence staining, take care to recover used primary and secondary antibodies. After recycling, it can usually be reused at least 5 times, and if there is abnormal phenomena such as turbidity and precipitation, it should be discontinued. A fluorescence microscope or high-content analyzer that can observe both green and blue fluorescence is required. The TRA-1-81 murine monoclonal antibody in this kit specifically recognizes an unknown antigen with a molecular weight of about 200 kDa on the surface of human or mouse pluripotent stem cells, and can be widely used in combination with fluorescence microscopy or high content screening (HCS), etc. , to determine the effect of iPS reprogramming, evaluate the stemness of pluripotent stem cells, and detect the differentiation of stem cells.