Plant Vesicle Rapid Extraction Kit

1. Before formal experiments，conduct preliminary tests with several samples to optimize experimental conditions and achieve optimal results. 2. Briefly centrifuge reagents in screw-cap microtubes before opening to collect liquid from the cap and tube walls at the bottom，preventing reagent loss upon opening. 3. Do not mix with reagents from other brands，as this may compromise performance. 4. Bacterial or fungal contamination of samples or reagents，or cross-contamination between reagents，may cause erroneous results. 5. Use disposable tips，tubes，bottles，or glassware whenever possible. Reusable glassware should be thoroughly cleaned to remove detergent and sterilized before reuse. 6. After completing the experiment，all samples and utensils that have come into contact should be treated according to specified procedures. 7. The number of vesicles varies greatly among different cell types. Select the sample quantity based on actual conditions，downstream applications，and literature references. Use as many cells as possible when conditions permit. 8. When harvesting cells，ensure that dead cells do not exceed 5%. Apoptotic/dying cells release numerous vesicles of varying sizes，which may contaminate vesicles derived from viable cells during extraction. 9. For electron microscopy analysis，isolated vesicle samples must be stored on ice or at 4°C for no longer than two days. No freezing，as this may compromise electron microscopy results. Plant Vesicle Rapid Extraction Kit is applicable for high purity total vesicles extraction from various plant foliage samples. By simple regular centrifugation instead of ultra-speed centrifugation，large amount of intact vesicles will be obtained from samples. This kit is rapid and efficient，saving more time and samples. The vesicles extracted have wide applications and can be used for subsequent experiments such as function study，protein analysis，RNA analysis，electron microscope analysis，and NTA particle size analysis. This kit is able to extract all vesicles with diameter ranging from 10 nm to 3000 nm. This kit is applicable for vesicle extraction from leaf，callus，or suspension cell samples from model plants such as Arabidopsis，tobacco，and rice. Applicable samples: Plants.