Plant Spermine Content HPLC Assay Kit

Product Description Spermine is a naturally occurring polyamine found in mammalian tissues， plants， bacteria， ribosomes， and bacteriophages. It is an important substance that promotes cell proliferation. Under acidic conditions， spermine exhibits polycationic polyamine properties and can bind to DNA in viruses and bacteria， thereby conferring greater stability and flexibility to DNA molecules. It is also an essential component of cell culture media. Spermine lacks ultraviolet (UV) absorption groups and fluorescent properties， and thus cannot be directly determined by conventional detectors. This kit employs a pre-column derivatization method: under alkaline conditions， dansyl chloride specifically reacts with the amino groups of spermine to form stable derivatives with UV absorption. The derivatives exhibit good characteristic absorption at 254 nm with high response， making them suitable for automated batch analysis by high-performance liquid chromatography (HPLC). This kit is specifically designed for the rapid and efficient extraction and quantification of spermine in plant samples. Instruments and Reagents Required for the Assay High-performance liquid chromatograph (HPLC) equipped with a C18 column (4.6 × 250 mm， water tolerance ≥ 90%) and a UV-Vis detector (VWD); ultrasonic cleaner; water bath; vortex mixer; electronic balance; low-temperature high-speed centrifuge (4°C， 8000 r/min); adjustable pipettes; syringe; organic membrane filter; HPLC-grade acetonitrile， etc.