Plant Methionine(Met)Content HPLC Assay Kit

Product Description L-Methionine is an essential amino acid that cannot be synthesized by the human body and must be obtained from food. It plays multiple key roles in vivo: as a methyl donor (generating S-adenosylL-Methionine， SAMe)， it participates in the methylation modification of biomacromolecules such as DNA and proteins; it is also a precursor for the synthesis of cysteine， thereby influencing the production of the important antioxidant glutathione. L-Methionine lacks ultraviolet (UV) absorption groups and strong fluorescent properties， and thus cannot be directly determined by conventional detectors. This kit employs a pre-column derivatization method: under weakly alkaline conditions， OPA (o-phthalaldehyde) and 3-MPA (3-mercaptopropionic acid) specifically react with the amino group of L-Methionine to form fluorescent derivatives. Upon excitation with ultraviolet light， these derivatives emit fluorescence at a longer wavelength than the excitation light. The fluorescence intensity is proportional to the L-Methionine concentration with a high response， making this method suitable for automated batch analysis by HPLC. This kit is specifically designed for the rapid and efficient extraction and quantification of L-Methionine in plant samples. Instruments and Reagents Required for the Assay High-performance liquid chromatograph (HPLC) equipped with a C18 column (4.6 × 250 mm， water tolerance ≥95%) and a fluorescence detector (FLD); ultrasonic cleaner; vortex mixer; electronic balance; low-temperature high-speed centrifuge (4°C， 10，000 r/min); mortar/homogenizer; adjustable pipettes; syringe; organic membrane filter; aqueous membrane filter; chromatographic-grade methanol， ethanol， and acetonitrile， etc.