Plant Isoleucine(Ile)Content HPLC Assay Kit

Product Description L-Isoleucine is one of the essential branched-chain amino acids (BCAAs) for humans， which cannot be synthesized by the human body and must be obtained from food. It is primarily oxidized and broken down in skeletal muscle to provide energy， bidirectionally regulates and stabilizes blood glucose levels， and works synergistically with leucine and valine to improve exercise endurance， maintain skeletal muscle metabolic homeostasis， and promote tissue repair. L-Isoleucine lacks ultraviolet (UV) absorption groups and strong fluorescent properties， and thus cannot be directly determined by conventional detectors. This kit employs a pre-column derivatization method: under weakly alkaline conditions， OPA (o-phthalaldehyde) and 3-MPA (3-mercaptopropionic acid) specifically react with the amino group of L-Isoleucine to form fluorescent derivatives. Upon excitation with ultraviolet light， these derivatives emit fluorescence at a longer wavelength than the excitation light. The fluorescence intensity is proportional to the L-Isoleucine concentration with a high response， making this method suitable for automated batch analysis by HPLC. This kit is specifically designed for the rapid and efficient extraction and quantification of L-Isoleucine in plant samples. Instruments and Reagents Required for the Assay High-performance liquid chromatograph (HPLC) equipped with a C18 column (4.6 × 250 mm， water tolerance ≥95%) and a fluorescence detector (FLD); ultrasonic cleaner; vortex mixer; electronic balance; low-temperature high-speed centrifuge (4°C， 10，000 r/min); mortar/homogenizer; adjustable pipettes; syringe; organic membrane filter; aqueous membrane filter; chromatographic-grade methanol， ethanol， and acetonitrile， etc.