Plant Aspartic Acid(Asp)Content HPLC Assay Kit

Product Description L-Aspartic acid is a non-essential amino acid for humans， as the body can synthesize it endogenously. However， additional supplementation may be required during special physiological stages such as growth and development. As an important excitatory neurotransmitter in the central nervous system， L-Aspartic acid participates in synaptic excitation transmission. It is also involved in the urea cycle metabolism， facilitating the conversion of toxic ammonia into urea for excretion from the body. L-Aspartic acid lacks ultraviolet (UV) absorption groups and strong fluorescent properties， and thus cannot be directly determined by conventional detectors. This kit employs a pre-column derivatization method: under weakly alkaline conditions， OPA (o-phthalaldehyde) and 3-MPA (3-mercaptopropionic acid) specifically react with the amino group of L-Aspartic acid to form fluorescent derivatives. Upon excitation with ultraviolet light， these derivatives emit fluorescence at a longer wavelength than the excitation light. The fluorescence intensity is proportional to the L-Aspartic acid concentration with a high response， making this method suitable for automated batch analysis by HPLC. This kit is specifically designed for the rapid and efficient extraction and quantification of L-Aspartic acid in plant samples. Instruments and Reagents Required for the Assay High-performance liquid chromatograph (HPLC) equipped with a C18 column (4.6 × 250 mm， water tolerance ≥95%) and a fluorescence detector (FLD); ultrasonic cleaner; vortex mixer; electronic balance; low-temperature high-speed centrifuge (4°C， 10，000 r/min); mortar/homogenizer; adjustable pipettes; syringe; organic membrane filter; aqueous membrane filter; chromatographic-grade methanol， ethanol， and acetonitrile， etc.